KRT8 antibody

Catalog No. ABIN112350

This anti-KRT8 antibody is a Mouse Monoclonal antibody detecting KRT8 in WB, IF, FACS, IHC (p) and IHC (fro). Suitable for Human and Zebrafish (Danio rerio).

Quick Overview for KRT8 antibody (ABIN112350)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: H1

Product Details anti-KRT8 Antibody

Specificity: This Monoclonal antibody H1 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells.

Cross-Reactivity (Details): Species reactivity (tested):Human, Zebrafish.

Purification: Purified

Immunogen: Cytoskeleton preparation containing Cytokeratin 8.

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunoblotting, Immunocytochemistry, immunohistochemistry on frozen andparaffin-embedded tissues and flow cytometry. Recommended dilutions: 1/25-1/200 for flow cytometry, and for immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Storage: 4 °C/-20 °C

Storage Comment: Store the original vial at 2-8 °C. After reconstitution store (in aliquots) at -20 °C.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8

Gene ID: 3856

NCBI Accession: NP_002264

UniProt: P05787